Two cryopreservation protocols and two llama semen dilutors

Difficulties in semen collection are due to high viscosity, the main factor that makes cryopreservation impossible. Two cryopreservation protocols (straws and pellets) and two commercial dilutors (AndroMed and Triladyl) were evaluated. Three 4-year-old male Q¿ara llamas, 9 sexually receptive females were selected, semen collection was performed by post-copulation vaginal aspiration, samples were evaluated, macro-microscopic characteristics of semen subsequently diluted with AndroMed and Triladyl independently, refrigerated from 38 to 5ºC in 2 hours, sperm motility and vitality were evaluated in the thawed samples. The post-thaw motility for the straw protocol was 41.17±15.46 and 43.24±14.61 % for AndroMed and Triladyl respectively, and for the pellet method it was 19.17±13.23 and 28.19±15.49 % for AndroMed and Triladyl, with a difference between methods (P<0. 01); for vitality for the straw method it had an average of 49.24±14.83 and 53.01±11.66 % for AndroMed and Triladyl, and for the pellet method it presented 28.60±10.89 and 42.68±13.38 % for AndroMed and Triladyl of sperm vitality.