Tumor Markers in clinical chemistry (Ca 125)

CA125 of homogenate obtained from post-menopausal patients with malignant ovarian tumor was partially purified by gel filtration technique. Two forms of CA125 were found (BI and BII) with molecular weights 670 and 100 kDa respectively. The binding characteristics of the partial purified CA125 with 125I-antiCA125 antibody were investigated. Kinetic parameters of the binding of 125I-antiCA125 antibody with CA125 of homogenate obtained from pre-menopausal patients with benign and malignant tumors, post-menopausal patients with malignant tumors, and partially purified CA125, at different temperatures were determined. The results indicated that the binding reaction was time and temperature dependent process and it follows second order rate law in all studied groups. The thermodynamics of the binding of 125I-antiCA125 antibody with CA125 of homogenate obtained from pre-menopausal patients with benign and malignant tumors, post-menopausal patients with malignant tumors, and the partially purified CA125, at different temperatures were determined. All studied groups showed exothermic and spontaneous reactions and the participation of enthalpy was low. Thermodynamic parameters of transition