Thermotolerant Bacterial Population in Allahabad Rivers, U.P., India

CHAPTER I: INTRODUCTION: Incidence of R-plasmids and their transmissibility in bacteria which harbor the genes for multi Drug resistance and heavy metal tolerance among bacterial population. CHAPTER II: REVIEW OF LITERATURE: Indicators of Microbial water quality, Development of indicators, Development of indicators, Key fecal indicator micro-organisms, Microbiological and Physiochemical Aspects of water quality, Antibiotic resistance among thermo tolerant bacterial indicators, Heavy metal tolerance among thermotolerant bacterial indicators, Plasmid mediated resistance in antibiotics and metal tolerance. CHAPTER III: MATERIALS AND METHODS: Geographical Location, Climatic Conditions, Sampling sites, Sample collection, Sampling of water, Physiochemical analysis, Bacteriological analysis: Bacterial strains, Antibiotics and Heavy metals, Molecular studies on Resistant¿Plasmid, Screening of transferable antibiotic resistance factors in vitro, Isolation of R+ Plasmids, Agarose-gel Electrophoresis, Physiochemical analysis of water, Isolation of thermotolerant coliforms, Identification of thermotolerant coliforms, Antibiotic Susceptibility Test, Metal tolerance test, Curing of antibiotics