Text Book of Tissue Culture Techniques

This Text Book acquires knowledge on Tissue culture techniques deals with the Preparation of tissue culture medium from commercial powder, the Role of serum in cell culture, Isolation of lymphocytes by density gradient centrifugation, Primary cell culture; Preparation of single cell suspension from spleen, Trypsinization of monolayer and subculturing, Cell counting and viability determination, MTT Assay for cell viability and growth, Preparation of metaphase chromosomes from primary lymphocyte culture, Cryopreservation and thawing, Isolation of RNA, Isolation of plasmid DNA and characterization, Isolation of DNA from tissues by phenol, Separation of the erythrocyte membrane, and Mitosis in root tips with technical aspects in detailed explanation.