Study Biofilm of Bacteria Isolates, Identified by16srRNA Gene Sequencing

Biofilm formation (slime production) plays an important role in the pathogenesis of infections caused by different microorganisms . Samples collected from denture and orthodontic devices were diagnosed genetically after cultured and diagnostic microscopy using 16SrDNA sequencing from both devices. 16SrDNA sequence database provides high quality excellent identification at the species and subspecies levels. Screened these bacterial isolates for ability to form biofilm formation using CRA test, TCP test and icaAD gene test found gene test 100% was the best test for identified bacterial species to form biofilm for both devices . Moreover, in the present study four bacterial strains were recorded in European Nucleotide Archive (ENA), National Centre for Biotechnology (NCBI) and GeneBank as new strains in world and registered in the name of Iraq / Basrah.