Qualitative Phytochemical Evaluation Of Three Savanna Plant Species

Extract of herbal plant sample which includes leaf, seed and fruit pulp of calotropis procera, garcinia kolaand adansinia digitata respectively were investigated for phytochemical content and tested against clinical isolates of mycobacterium tuberculosis (in-vitro) and determination of minimum inhibitory concentration. 95% methanolic extract of the plant samples were obtained by percolation. The antomycobactarial activity was determined using disc diffusion method; anova single factor was statistically used. The crude methanol extract of calotropis procera leaf, garcinia kola seed and adansonia digitata fruit pulp reveal a final weight of 8.1g, 19.1g and 7.8g respectively, tannins and flavoniods were the phytochemical compounds found to be present in all the plant samples. Rifampicin antibiotic was used as control and the antimycobacterial activity for the plant sample shows that c. Procera has the highest zone of inhibition followed by g. Kola then lastly a. Digitata, the minimum inhibitory concentration ranging from 500-2000ug/ml. The m.I.C of 95% methanol extract in the order mention above are 500-2000ug/ml, 1000-2000ug/ml and 2000ugml respectively.