Purification and characterization of RfBP from coot, eagle and hen

Vitamin carrier proteins capable of high affinity interaction with their respective vitamins are present throughout the animal kingdom and play a vital role in the life processes of the vertebrates. The biological significance of these specific proteins include storage & transport of vitamins and preventation of rapid losses of these vital nutrients due to exertion and metabolic degradation. These carrier proteins stoichiometrically and reversibly bind the vitamins with high affinity and receptor like specificity. Vitamins are known to remain biologically inert as long as they are associated with their carrier proteins and can be activated only upon dissociation. The specific interaction of vitamins with their respective carriers is through non covalent forces, thereby permitting reversible dissociation of the unmodified ligands from the carriers under appropriate physiology conditions.