Performing Enzymatic Reactions Using Novel Polymer-Bound ATP

the present work was devoted to develop an efficient synthetic process which requires cofactors. The enzymatic glucose 6-phosphate (G6P) production from glucose was demonstrated as a model process. As cofactors, two new polymer-bound adenine nucleotides (ATP and ADP) were synthesized using starburst polyamidoamine (PAMAM) dendrimers. Then, the kinetics of the reactions catalyzed by Bacillus stearothermophilus glucokinase (GK) and acetate kinase (AK) using these polymer-bound cofactors were studied extensively. Finally, the production of G6P using the polymer-bound ATP in a plug flow-type hollow-fiber membrane reactor (PFMR) in a diffusion mode as well as a continuous stirred tank-type membrane reactor (CSTMR) in a filtration mode was investigated theoretically