PCR investigation of ocular infections caused by Candida sp

A wide variety of fungi is known to cause human ocular infections. Candida is widespread yeast not directly linked to environmental factors for ocular infections. Candida is the most common ocular fungal pathogen and can also causing diseases such as conjunctive, lacrimal drainage system and retina.Coding rRNA genes are particularly attractive targets because multiple copies are present in each genome and the genes have conserved regions for designing broad-range primers and have more variable regions for identifying fungi. Most studies have focused on regions within the 18S rRNA gene, the 5.8S rRNA gene and 28S rRNA gene for developing broad-range PCR primers targeting human fungal pathogens. Recently, several complete fungal genomes have been sequenced.The sequences were compared to reference data available at the Gene Bank databases by using BLAST (basic local alignment search tool) to determine sequence identification.