Non-target metabolomics in the analysis of biodiverse fruits

Two fruits recognized for their functional properties, tropical mountain blackberry (Rubus adenotrichos) and noni (morinda Citrifolia) have been the subject of nutritional intervention studies in humans and in Wistar rats. A non-targeted metabolomic approach was implemented based on mass spectrometry analysis (UPLC-DAD / ESI-Q-TOF-MS) of urine collected just after ingestion of the juices and following short daily consumption. term. The objective is to determine the main urinary metabolites resulting from the consumption of these fruit juices and to deduce the potential health effects. In the absence of commercial standards, the attempted identification of metabolites has mostly relied on determining the exact mass and comparing the ion fragmentation spectra, listed in international databases.