Newcastle-Avian flu recombinant vaccine in embryonated eggs and chicks

Newcastle and avian flu virus consider two of the main avian plagues, which can destroy not only chicken industries but also birds industries and alter the bird eco-system. The main problem with both virus, they associated with a high degree of mortality in velogenic strains and the difficulties to differentiate between infected and vaccinated birds and sometimes the bird get infected before the vaccine works as most routine vaccination approaches based on vaccination of hatched chicken not based on vaccination of the embryonated eggs, so this work has been done, where it provided data for a vector vaccine, easy to differentiate between infected and vaccinated and provided accepted, low cost, time saving approach and provided data about the protection from one day old chick, where we provided data about protection levels after embryonated eggs vaccinations against two of the most important viral diseases in chicken, which provided immunized one day old chick with immune level able to protect against virulent infection with high percentage for both avian flu and Newcastle viruses.