My Battle in the Biochemical Biotin Study with an HPLC as Katana

Kou Hayakawa is a Biochemist possessing the technology of high-performance liquid chromatography (HPLC). Recently, simple and quantitative HPLC-photometric assays have become indispensable tool to study the biochemistry, biology, and cancer biology. He has developed HPLC determination methods for protein, polysaccharide, and enzyme activity, and has invented the quantitative protein-direct-microsequencing-deciphering (PDMD) method, which quantitatively identify the expressed proteins in the biological specimens via determining phenylthiohydantoin (PTH) amino acids derived from the Edman degradation reaction (automated protein-microsequencer). This technology has been applied on hepatocellular carcinoma (HCC), and the origin of cancer has been identified to be the co-expression of the Gag polyprotein of human immunodeficiency virus (HIV-1; with reverse transcriptase (RNA-dependent DNA polymerase (RdDP)) and integrase (IN)) and the Genome polyprotein of human hepatitis virus (HCV; with RNA-directed RNA polymerase (RdRP)). Then, this book is useful for professional biological researchers and anyone else who may be interested in the cancer.