Molecular Detection of AmpC gene families Encoding for Antibiotic

Our study was designed to detect and evaluation Ampicillin class C B- lactamases genes from Escherichia coli isolates in patients with UTI by using of PCR technique . A total of 130 (52%) samples with significant bacteriuria were detected. The study showed higher incidence of UTI in females (73.08%) than males (26.9%).The bacteria which were grown on culture of all the 130 urine samples with significant bacteriauria were as follows: 60 ( 46.2 %) isolates of E. coli and 70 ( 53.8% ) were other isolates such as P. aerogenosa , Proteus mirabilis , K. pneumonia and Staphylococcus aureus. The results showed that only 18 (34%) isolates gave positive results with bla AmpC gene and all bla AmpC positive isolates showed diversity presence of plasmid-mediated AmpC B-lactamases. The genes t h a t encoded FOX,CIT, DHA, EBC, ACC and MOX were found in the percentage of 44.4, 38.9, 27.8, 50.0, 22.2 and 27.8, respectively.