Molecular Characterization of Fusarium sp. Using SCAR Markers

This book deals with development of putative SCAR markers from monomorphic bands of RAPD and ISSR primers.The primers used for identification of Fusarium oxysporum f.sp.ciceri were ISSR (GA)8YT ,(ATG)6,(GA)8T) and RAPD, OPA13, OPA18 and OPB14. The races selected for studies were Type Race-1 (Hyderabad and Akola), Type Race-2, (Kanpur), Type Race-3, (Gurdaspur) and Type Race-4 (New Delhi and Jabalpur). These primers showed the monomorphic bands, these can be used for development of the putative of SCAR (Sequence Characterized Amplified Regions) markers for identification of Fusarium oxysporum f.sp.ciceri. The amplicon size 600 bp(ATG)6, 700 bp(GA) 8YT and 650 bp of ISSR and 750 bp(OPA18),1.4 kb (OPB14),1500 and 800 bp of OPA 13 were eluted, isolated, confirmed and sequenced. The primers were designed with the sequenced information of these two fragments using primer 3 software and putative SCAR markers were developed. Races 1 and 4 showed close virulence patterns on chickpea differentials.