Molecular Breeding and Genetic Resources of Tulaipanji Rice

This book deals with the various aspects of SNPs/Indels variation in the progeny lines (Tulaipanji x Ranjit) and genetic resources of wild rice Oryza rufipogon. Rice germplasms have been described based on morpho-physicochemical properties, GCMS analysis, SEM of grain and starch granules. Reverse genetic based TILLING/Eco-TILLING technology has been illustrated for allele mining. NGS based GBS method (Illumina) was employed in this study to analyze the SNP and InDel variations and their introgression pattern into F5 progeny lines (P-awn and P-awnless). Total no. of SNPs identified was 52,810 and Indels 4327. These variants were not evenly distributed throughout the 12 chromosomes (based on Golden Helix G Browser). Study showed that out of 10013 allelic count, 92.52% was introgressed into P-awn from Tulaipanji and 7.485 from Ranjit, in contrast, P-awnless carried 89.19% from Ranjit and 10.81% from Tulaipanji. It is due to segregation distortion. Many SNP variations are associated with important traits. Such as GA20-oxidase (sd1 gene), Argonaute and Dicer protein with PAZ domain. Fastq files of four rice lines with NCBI SRA Accession no. SRP077861, SRP077919, SRP077947 & SRP077977.