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Beschreibung
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Details
| Verlag | Springer Netherland |
| Ersterscheinung | 14. Dezember 2014 |
| Maße | 23.5 cm x 15.5 cm |
| Gewicht | 219 Gramm |
| Format | Softcover |
| ISBN-13 | 9789400795266 |
| Seiten | 126 |
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