Micropropagation of Guizotia abyssinica Cass.

Micropropagation Of Guizotia Abyssinica Cass. Sushila Devi Shrestha and Sanu Devi Joshi Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal Guizotia abyssinica Cass. is an important oil-yielding crop commonly known as Niger. It is generally sown as a mixed crop with different grains and pulses. Its propagation and domestication is not considered satisfactorily. The morphogenetic responses and totipotency of various vegetative parts were studied. Murashige and Skoog (1962) medium (MS) was used as the basal medium (BM). The explans excised from the seedlings grown in vitro. Various vegetative parts such as root, stem, leaf, hypocotyls, nodal part and shoot tip showed their totipotency for calli proliferation and differentiation into roots, embryoids, shoot buds and plantlets. Callus proliferation was more effective in the presence of hormones in the medium. Forty percent of excised shoots differentiated roots when they were transferred to in vivo. Rooting in vitro, MS liquid media and MS media with rooting hormones were recorded the best. The plantlets raised were acclimatized.