Mapping of RAPD primers on chromosome 2A of common wheat

Common (bread) wheat is an allohexaploid species (Triticum aestivum L.) with 3 compensating genomes AABBDD (2n=6x=42 chromosomes). Traditionally genetic and physical mapping in this most important cereal crop has been based on molecular markers (RFLPs, PCR, Insitu hubridisation etc). The recent development in producing deletion lines of common wheat (using Aegilops cylindricum genome) has revolutionized the physical mapping of wheat chromosomes. During present study, deletion lines of chromosomes 2A of common wheat were used to identify RAPD (Randomly Amplified Polymorphic DNA) based molecular markers specific for short and/or long arm of chromosome 2A. Out of the 7 RAPD primers used, OPA-07 showed useful polymorphism. By comparing the C-banding karyotype of the deletion lines, it is inferred that the primer (OPA-07) anneals to the distal half of the long arm of chromosome 2A.