Lipase production from haloalkalophilic organisms

A study was conducted for isolation and characterization for lipases(cheap and versatile catalysts to degrade lipids in more modern applications) from hyperalkaline and hypersaline soil. Amongst 15 isolated strains, 9 isolates were screened for lipase production and further study was followed by screening of best lipase producers. In addition, lipase production was also measured in presence of various parameters viz. different lipidic carbon sources, metal ions, temperature, pH to optimize the medium. Efforts have been made to purify the protein by ammonium sulphate precipitation, gel filtration, and ion exchange chromatography. However, in the future we can consider genetical characterization and selection of the most desirable strains, which can assess their potential as starter cultures for commercial use.