Isolation, identification and genetics of nylon6 degrading P. putida

Rapid developments in the chemical industry have lead to the distribution of a wide variety of synthetic compounds into the environment. Synthetic polymers form the base for the more than 55% of all textile material with a worldwide fiber production of 3.3 million tones. Research on the microbial degradation of xenobiotic polymers has been underway for more than 40 years. It has exploited a new field not only in applied microbiology but also in environmental microbiology and has greatly contributed to polymer science by initiated the design of biodegradable polymers. According to important use of nylon, and because of limited studies of nylon biodegradation, this study was focused on: ¿ Isolation and identification of bacteria that capable of degrading nylon6. ¿ Screening the bacteria for their ability to degrade nylon6 and select the efficient isolate(s). ¿ Determine the plasmid(s) profile of the efficient isolate(s). ¿ Determine the role of plasmid(s) in nylon6 degradation process via curing and/or transformation experiments. ¿ Study some optimum conditions for nylon6 degradation by efficient isolates.