Investigation of in vivo interactions between the Dsl1 tethering complex and COP-I vesicles, and functional implications for retrograde trafficking

A unique feature of eukaryotic cells is their compartmentalization into organelles with specialized structure, function and composition. This ensures the execution of many distinct cellular processes within different micro-environments. However, with this separation, certain complications arise. Cells have had to develop mechanisms by which organelles exchange material (trafficking). Additionally, cells need to ensure that during growth and division, organelles are passed on to their progeny (inheritance). In the interplay between endoplasmic reticulum (ER) and Golgi apparatus, diverse processes are involved in trafficking and inheritance. The budding yeast Saccharomyces cerevisiaeis a versatile model organism to study these intracellular processes.