In Vitro propagation of plant through liquid culture system

The traditional propagation methods allow the clonal multiplication of genotypes of interest but at relatively low propagation rates, which explains why the introduction of a new genotype into agricultural practices may take a number of years. Tissue culture technique has emerged as best alternative tool before three decades to overcome this problem. It allows the rapid production of a large number of plants, even where normally the species has low multiplication rates. At the same time, the space requirement for such multiplication is considerably smaller. This application is therefore ideal for the massive multiplication of species or genotypes with commercial potential, whether these are plants of agriculture or horticulture, such as fresh cut flowers, ornamental plants or fruit trees. The massive propagation of plants has traditionally been carried out in solid medium nevertheless during the last few years; cultures in liquid medium with the objective of massive plant propagation have appeared as an alternative, which allows reduced costs in relation to numbers of subculture and losses associated with manipulation and contamination, but also because of space reduction.