In vitro plant regeneration

The experiment was conducted at Advanced Plant Breeding Laboratory, Department of Genetics and Plant Breeding and Tissue Culture Laboratory, Department of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh. Dehusked seeds of five aromatic rice varieties viz. Radhunipagol, Benaful, Guamuri, Thakurbhog, Padmabhog and Pokkali (used as a control rice variety) were placed on MS media supplemented with three salts (NaCl, Na2SO4 and KCl) at four different levels (0, 2.5, 4.5 and 6.5 g/l). About 3-6 weeks after, the calli were transferred to MS media for regeneration. Out of six varieties, Radhunipagol showed better performance for callusing at NaCl salt and the highest plant regeneration was recorded in Padmabhog with Na2SO4 salt. The value of parameters declined with the increase of salinity levels. Radhunipagol (97%) performed best for callus induction with NaCl salt at 2.5 g/l but Padmabhog (65.36%) showed best regeneration with Na2SO4 salt at 4.5 g/l. So, it is stated that Radhunipagol is more potential variety for callusing against NaCl salt at 2.5 g/l and Padmabhog is most potential variety for regeneration against Na2SO4 at 4.5 g/l.