In vitro micropropagation of Brahmi (Bacopa monnieri)

The study entitled ¿Effects of growth regulators on in vitro multiplication of Bacopa monnieri¿ was carried at Centre for Pant Biotechnology (CPB). The results obtained are summarized below: ¿ The optimum time period of sterilization with no contamination and 100% survival for shoot tips is two minutes using 0.1% HgCl2. ¿ The zero contamination with maximum survival rate was obtained when nodal segments were treated with 0.1 % mercuric chloride for three minutes. ¿ Maximum regeneration in shoot tips and nodal segment was observed at minimum concentration (0. 25 mg/l) of auxins i.e. IBA and NAA. ¿ The optimum concentration of IBA and NAA is 0.25 mg/l for each. ¿ BAP and Kinetin were used alone maximum numbers of shoots were formed at the concentration of 1.0 mg/l. ¿ Both the cytokinins (BAP and Kinetin) were used in combination the best results were obtained in BAP (1.0 mg/l) + Kinetin (0.2 mg/l) followed by BAP (0.2 mg/l) + Kinetin (1.0 mg/l). ¿ Lower concentrations (1.0 & 0.2 mg/l) of cytokinins (BAP and Kinetin) either used alone or in combination shows good results.