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Many of the Pattern Classification Problems in Bioinformatics can be addressed by various Artificial Intelligence techniques.Pattern classification is the study of how a computer can perceive the environment and investigate how it can differentiate the patterns in their respective fields, and make intelligent actions based on the type and categories of the pattern.This book gives the importance of Artificial Intelligence in solving major Bioinformatics problems . It has lots of examples and case studies. The basics of AI and Bioinformatics was provided initially.The thinking of why AI is raised in the next section.The actual plan of action was given in a detailed manner.I thank the Management, Principal, Vice Principal of Shri Vishnu Engineering College for Women for their encouragement in publishing this book.