Identification of Bacillus producing thermotolerant hydrolases

Thermotolerant enzymes were produced from soil and water Bacillus species as, protease (89.28%) (73.17%), amylase (55.35%) (51.21%), lipase (91.07%) (95.12%), cellulase (85.71%) (60.97%) and chitinase (85.71%) (92.68%), respectively, after culturing on solid agar media with different incubation period, at 50°C. So, 41.23% of soil and water Bacillus isolates were detected for hydrolases production as (protease, amylase, lipase, cellulase and chitinase) together, and showed that B. polymyxa strains were the best bacteria in protease, amylase and cellulase production, whereas B. lentus strains was a good chitinase producer. Lipase was produced by several Bacillus strains as B. circulans, B. stearothermophilus, B. polymyxa, B. mycoides and B. lentus, after culturing on different media and incubation period, at 50°C.