Genetic diversity of Phlebotomus orientalis in Sudan

This study was carried out elucidate genetic variation among populations of Phlebotomus orienanlis vector of visceral leishmnaiasis from three different geographical regions in Sudan. Sand flies were collected from White Nile, Gedarif, Blue Nile and Khartoum States using CDC and sticky paper traps. Specimens collected from these areas were analysed using Rapid Amplified Polymorphic DNA -Polymerase Chain Reaction(RAPD-PCR). RAPD-PCR was performed using 30 random primers. Population of each site investigated showed a characteristic diagnostic DNA bands. Furthermore, of P. orientalis showed different level of diversity at RAPD loci. In addition, clear clustering of P. orientalis based on their geographical regions were observed.