Genetic Divergence, Induced mutation and Interrelationships

Genetic diversity was assessed using 15 morphological characters. Based on multivariate (D2) analysis, 60 mango genotypes were grouped into 8 clusters. Three enzymes viz., gulutamate oxaloacetate transminase (GOT), malate dehydrogenase (MDH) and peroxidase (PER) were used to investigate genetic diversity of 60 mango genotypes at protein level. Eight zymotypes with GOT, 10 with MDH and 7 with PER were formed by 22, 39, 12 bands, respectively at different Rf values. Compared with three studied DNA extraction protocols of mango such as SDS, CTAB and water saturated ether (WSE) method with NaCl, it was found that WSE method with NaCl had highest value of average percentage (85.44%) in DNA content of the mango genotypes. Study of RAPD for 60 mango genotypes was done to detect genetic diversity at DNA level. Of 40 primers, 11 decamer primers amplified 104 bands. The mean genetic diversity among all the accessions was 0.323 and Shannon's information index was 0.489 across all loci. Induction of mutation on 8 selected genotypes derived from morph-molecular diversity study was performed by applying three doses of radiation such as 20 Gy, 30 Gy and 40 Gy.