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Folding Pathways of Photoactive Proton Pump Bacteriorhodopsin

Folding Pathways of Photoactive Proton Pump Bacteriorhodopsin

von Amjad Farooq
Softcover - 9783838319438
59,00 €
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Beschreibung

Stop-flow fluorescence and photodiode array spectroscopy, with a millisecond time resolution, are used here to investigate refolding kinetics of the photoactive proton pump bacteriorhodopsin in mixed DMPC/CHAPS micelles from a partially denatured state in SDS. The study suggests that both the apoprotein folding and subsequent binding of retinal chromophore likely proceed via distinct multiple parallel pathways to generate the native helical bundle. Taken together, these data should have profound implications on the mechanisms of folding of proteins within biological membranes.

A detailed kinetic study involving stop-flow spectroscopy to decipher the folding pathways of Bacteriorhodopsin

Details

Verlag LAP LAMBERT Academic Publishing
Ersterscheinung 11. Januar 2010
Maße 22 cm x 15 cm x 1 cm
Gewicht 244 Gramm
Format Softcover
ISBN-13 9783838319438
Seiten 152

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