Femtosecond laser surgery of the crystalline lens

PART I : Review Knowledge of femtosecond laser used on crystalline lens. PART II : Study Purpose: the aim of this study was to analyze the interactions during femtosecond Laser photodisruption into ex-vivo porcine crystalline lens and to study parameters for Laser interaction optimization Methods: an experimental femtoseconde laser was used. The laser characteristics were: 1030 nm wavelength, pulse duration 400 fs and numerical aperture 0.13. A specific software was created to custom and monitor any kind of photoablation patterns for treatments. Porcine crystalline lens were placed into an open sky holder filled with physiological liquid (BSS®) and surmounted by a glass plate. A numerical camera was associated with a metrological software in order to magnify and quantify the results. Transmission Electronic Microscopy (TEM) was performed in some samples to identify the microscopic plasma interactions with crystalline. The optimization of parameters was investigated in term of optical breakdown threshold, sizing of interactions and best pattern for alignments. More than 150 crystalline lenses of freshly enucleated pigs were treated.