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The gold nanoparticles prepared by laser ablation (Q-switched Nd:YAG) at different experimental parameters. The results show that, as the ethanol ratio increases, the average size of prepared GNPs was increased and their size distribution became broad. The second parameter studied was the ablation time. As ablation time increased, the concentration of the obtained GNPs was increased. If the fluence of laser beam increased, the average size of particles became smaller until, it reaches to critical size at certain fluence and then became increase again as the fluence increased. It was found that the 1064nm wave length was more effective than 532nm wavelength. When the geometry of laser beam changed the shapes and the average size of GNPs were changed.