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E. coli Plasmid Vectors

E. coli Plasmid Vectors

Softcover - 9781617373916
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Beschreibung

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Methods and Applications

Details

Verlag Humana Press
Ersterscheinung 10. November 2010
Maße 23.5 cm x 15.5 cm
Gewicht 505 Gramm
Format Softcover
ISBN-13 9781617373916
Seiten 316

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