Development of Functionalized Derivatives for the Application in a Novel Bioorthogonal Ligation Strategy and for the Design of IHF Mimicking Peptides

Proteins are involved in countless cellular transformations. Owing to their diverse and significant role in biological systems, biologically relevant macromolecules have gained attention as target structures in order to elucidate their functions and to design novel bioactive materials with respect to medical applications. [l,2l Especially, site selective functionalizations of proteins allow the examination of the special and temporal localization, dynamics and function of biologically active samples in a cellular environment and more importantly even at the molecular level. l31 However, in many cases selectively functionalized proteins are difficult to access by applying recombinant techniques as these are limited to genetically encoded amino acids and site directed modifications are often impossible to control.l11