Determining the Frequency of Some Human Molecular Markers

DNA is found in any human tissue, making it easy to obtain. Experiments carried out in 1985 by Jeffreys and collaborators revealed repeating units of DNA that vary in number between individuals. STR sequences have repeating units varying in size between 2 and 7 base pairs and constitute microsatellite regions of DNA. CODIS, or the Combined DNA Index System, was the first coded DNA database founded by the FBI from the use of molecular markers and is universal, containing 13 to 21 STR genes with known frequencies in different population groups and used for Paternity Tests and forensic DNA. The aim is to standardize and implement the molecular markers belonging to the CODIS system. Analysis of allele frequencies and comparison with other populations, for implementation of the Paternity Test at the Federal University of Rondônia (UNIR). Primer sequences form Multiplex subsets as described in the literature.