Complete Process of Agrobacterium Mediated Gene Transfer in Tobacco

Agrobacterium tumefaciens is a remarkable species of soil - dwelling bacteria that has the ability to infect plant cells with a piece of its DNA. Plant transformation mediated by A. tumefaciens has become the most used method for the introduction of foreign genes into plant cells & the subsequent regeneration of transgenic plants. Tobacco is a tall leafy annual plant that belongs to the Solanaceae family. Every part of the plant except the seed contains nicotine, but the concentration was related to different factors such as species, type of land, culture or weather conditions. Annexins are soluble, hydrophilic proteins capable of binding to the negatively charged membrane. From the result of the present research, it was concluded that the A. tumefaciens mediated transformation in tobacco was a successful method for transfer of annexin gene. The methodologies can also used for the commercial production of transformed tobacco with salt & drought tolerance. Undoubtedly, the development of transformation procedures based on A. tumefaciens - mediated gene transfer for new economically important species are advisable and the results obtained in recent years evidence a promising future.