Bone Research Protocols

165,84 €
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von Miep H. Helfrich, Stuart H. Ralston, Stuart H. Ralston (Hrsg.)


Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.

Tags: Biologie, Mikrobiologie

Hardcover - 9781617794148
Auflage: 2nd ed.
Verlag: Springer-Verlag GmbH
Ersterscheinung: November 2011
ISBN-13: 9781617794148
Größe: 266 mm x 189 mm x 41 mm
Gewicht: 1361 Gramm
643 Seiten

Taschenbuch - 9781493961009
Auflage: Softcover reprint of the original 2nd ed. 2012
Verlag: Humana Press
Ersterscheinung: August 2016
ISBN-13: 9781493961009
Größe: 254 mm x 178 mm x 35 mm
Gewicht: 1218 Gramm
660 Seiten
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