Biotoxicity assay of Bacillus thuringiensis

It is concluded that present research is very reliable and consistent in providing the ways of establishing careful inventions in controlling stored pests. The Cinnamum aromaticum can be rate effective for groups to control stored product insect pests i.e., T. castaneum. Blending of B.t. isolates with diverse botanical compounds like cinnamon in a definite proportion can be supportive in making bio-pesticide product. The lethal concentration (LC50) of cinnamon powder was 0.4 g/g against second instar larvae of T. castaneum. Results were notable in case of larvae. When compound was mixed with three isolates of B.t. then toxicity was increased. B.t. is famous for its genetic content as well as pesticidal role. Bio-toxicological effect was evaluated by combining three isolates of B.t. and cinnamon powder against larvae and adults of T. castaneum. High concentrations showed high mortality rate and less with the passage of time. This is a new study in which combining together all the essential elements are very effective against T.castaneum. All elements are safe for humans and animals. Their synergistic effect can not only control stored target pest but also non-target