Biochemical and Biophysical Methods in Molecular and Cellular Biology

This book focuses on the fundamental principles and applications of several modern biochemical and biophysical techniques employed in molecular and cellular biology. It describes cutting-edge techniques for studying single molecules/biomolecules, subcellular structures, and cells. The book chapters provide an in-depth understanding of methods currently employed to visualize and probe molecular and cellular processes. The techniques discussed in this book include Mass spectrometry, Microscopy techniques, Forster resonance energy transfer (FRET), Z-scan, Fluorescence correlation and cross-correlation spectroscopy, Dynamic light scattering (DLS), X-ray crystallography, Total internal reflection fluorescence (TIRF) microscopy, Cryo-EM, NMR spectroscopy, Optical tweezers, Magnetic tweezers, Raman spectroscopy, Atomic force microscopy (AFM), Optogenetics, bioinformatics, etc. The book chapters also include the biomedical, industrial, and R&D applications of these methods. Also included are sections on data analysis and its interpretation. Overall, this book offers a comprehensive and detailed understanding of several modern techniques in molecular and cellular biology.