Spin Column Designing for DNA Extraction

The focus of the study is the spin column design with different materials. Effects of the binding of DNA with silica, glass microfiber membrane, whatmann filter paper under different binding conditions are discussed. Silica-based media and glass fiber membrane have become the standard for DNA binding in the plasmid preparation process. Chaotropic salts facilitate binding of DNA to the media. This creates a convenient way to wash away contaminants prior to eluting the DNA with a neutral solution, such as TE Buffer or water. Filter paper pellets can easily be processed by vacuum filtration or centrifuge to yield desired quantities of DNA. The use of spin column is a rapid, economic and sensitive method for DNA isolation. The use of these extraction method is both time and cost saving. Average, standard deviation, and coefficient of variation have been calculated for three methods.