Production of Laccase by Pleurotus Ostreatus Using Coconut Shell

Laccases are among the group of multicopper oxidases with a broad substrate range and specificity. They use one electron to oxidise molecular oxygen and reduce it to water. Laccase's catalytic process uses molecular oxygen as the final electron acceptor. This enzyme can perform electron transfer processes very well due to the capacity of these copper atoms to flip between their oxidation states. Laccase (p-diphenol: oxygen oxidoreductases; 1.10.3.2) is the glycoprotein, which oxidises diphenols using oxygen molecule as acceptor of electron and are classified as blue oxidases by the Enzyme Commission. These enzymes catalyse the oxidation of a wide range of substrates, including aromatic amines, methoxy phenols, mono, di, and polyphenols, aminophenols, and ascorbic acid. These enzymes are monomeric in form and include 15¿30% carbohydrate having a molecular mass of 60¿90 kDa.