Polymerase Chain Reaction

Nowadays, advances and applications of research in biochemistry and genetic play an important role in the field of health sciences. This has become necessary a molecular approach of the disease for a better interpretation of processes and as horizon in the development of new diagnostic and therapeutic strategies. Therefore, techniques in molecular biology have modified diagnosis, prevention and control of diseases in living beings. The polymerase chain reaction (PCR) is a primer-mediated enzymatic amplification of specifically cloned or genomic DNA sequences. This PCR process, invented more than a decade ago, has been automated for routine use in laboratories worldwide. Diagnosis of disease now a days are mostly laboratory dependent. Due to recent advances in medical science and molecular biology, most of the diagnosis of uncommon, complicated, unusual presentation of disease has left the option of molecular diagnosis as the number one diagnostic modalities. Many molecular techniques are now being widely used throughout the world including PCR, flow cytometry, tissue microarray, different blots, and genetic diagnosis. Among these PCR is the most widely accepted and commonly used.