Pea Rhizobium Biochemical test

Under the study of taken bacterial isolate and characterize the performance of Rhizobium leguminosarum bv viceae strains respect to tolerance to temperature, pH and production of indole acetic acid(IAA), Phosphate solubilization test from pea plant collected in the surrounding areas of different districts of Uttar Pradesh, India. Temperature and pH is an important abiotic factor, which directly affects growth, enzyme activity, metabolic activity, root hair infection, asteroid differentiation, nodule formation and structure, the functioning of the legume root nodule and nitrogen fixation in pea Rhizobium isolates . Above the optimum temperature, infection and nodule growth is slowed down so that a smaller aggregate amount of a less active tissue is formed and symbiotic effectiveness is reduced. All property also effected by IAA production and phosphate solubilization activity of characterized bacteria isolated from pea (Pisum sativum) plants.