Optimal Pre-treatment and Resolubilisation in Enzyme Solutions

This book will discuss how optimization of physical parameters (e.g., temperature, maturation time and pH) related to protein solubility affect the cost of the total process of the enzyme production at industrial scale. The influence of physical parameters and chemical pre-treatment on the solubility of proteolytic enzyme; Protease A and B are discussed in details. Protease A and B, are recombinant proteolytic enzymes produced from genetically engineered Bacillus licheniformis was used in this thesis work. This study clearly demonstrates that optimization of some physical parameters and chemical pre-treatment improves the solubility of proteolytic enzyme and thus establishes the conditions of parameters in a cost-effective strategy of enzyme recovery and production at industrial scale.