Histomorphometric, Histochemical and DNA Quantitative Analysis of OSCC

The objective of the present study was designed to establish more specific criteria for the degree of differentiation and nuclear DNA histochemical quantitation of OSCC. Morphometric measurements by image analysis computer system and DNA histochemical quantitative analysis were assessed in association with clinicopathological features of OSCC. Two histological grading systems were used; Broders' grading system & Invasive front grading system using four histomorphological features that includes; degree of keratinization, nuclear pleomorphism, pattern of invasion and host response (inflammatory cell infiltration). The morphometric measurement was carried out using H&E staining techniques. Each nucleus and cell was submitted to morphometric parameter measurements that include nuclear area, maximum diameter (D max), form AR and D circle, cellular morphometric perimeter and nucleo: cytoplasmic ratio. A microspectrophotmerty technique was used to determine the quality of tumour nuclear DNA content with both histological grading systems of OSCC that stained by Feulgen reaction. Esterases histochemistry was employed to evaluate the distribution of carboxyl ester hydrolases in OSCC.