Development and Validation of New Spectrophotometric and HPLC Methods

Advantages OF HPLC By using HPLC we can separate more than 200 compounds from a mixture. Ready applicability to accurate quantative determination.Suitability for separating volatile and nonvolatile species.Faster Analysis with higher resolution.Greater reproducibility due to close control of the parameters affecting the efficiency of the separation.We can analyze up to Nano gram to Pico gram level also.Easy automation of instrument operation and data analysis.Applicability to large scale, preparative procedures.Applies high pressure to solvent flow.HPLC columns can be reused without repacking or regeneration.Applicability of wide linear dynamic range.HPLC System is robust for small changes.Drugs of abuse in urine, blood, tablets etc. analysis can be done.