Craniofacial Development

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face ( c. elegans , zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and  in vivo  cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using  in-situ  hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful  Methods in Molecular Biology  series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.