Cloning, heterologous expression and partial purification of IF2

It is important to evaluate the role of the IF2 within the bacterial translation regulatory mechanism as well as to pose the IF2 as a molecular target for repurposed non-steroidal anti-inflammatory drugs (NSAIDS). The aim of this project is to identify a novel and an effective therapeutic endogenous molecular target in Mycobacterium tuberculosis. In this study, I have identified, cloned, expressed and partially purified the novel putative endogenous therapeutic target for various non-steroidal anti-inflammatory drugs (NSAIDs) from H37Rv strain of Mycobacterium tuberculosis: putative mycobacterial infB, Rv2839c encoded protein which is putative protein translation initiation factor 2 (IF2) in a few species of mycobacteria. I conclude it may lead us to the discovery of the new anti-tubercular role of some old inexpensive NSAIDs and/or their derivatives as effective drugs against IF2 of Mycobacterium tuberculosis.