Application of Immobilized Laccase in Bioreactor

Immobilization of enzyme was conducted in two methods, entrapment by calcium alginate and agar-agar with maximum immobilization efficiency 49% and 32%, respectively and covalent method by chitosan and gelatin with maximum immobilization efficiency 63% and 50% respectively. Decolorization of black textile dye was performed also by operation of packed bed bioreactor continuously at different flow rates (15, 30 and 45) ml/hr and HRT (7.9, 3.9 and 2.6) hr. respectively. The black textile dye was loaded over the chitosan immobilized laccase with total working volume of 118 ml at concentration of (30 mg/l) in pH 5.0 at 30oC. Decolorization efficiency of black textile dye with immobilized laccase in a bioreactor increased at flow rate 15 ml/ hr., and reached to 71% after 2.5 hr. While the increase in flow rate to 30 and 45 ml/ hr. resulted in dropping of decolorization rate of dye to 60 and 47% respectively after period of time.