{"product_id":"vsv-pseudotypes-bearing-hbsag-von-manujendra-saha","title":"VSV Pseudotypes Bearing HBsAg","description":"\u003cp\u003eAnalysis of hepatitis B virus (HBV) infection has  been difficult due to a lack of suitable culture  system. Pseudotyped viruses provide a safe viral  entry model because of their inability to produce  infectious progeny virus. Here a system has been  developed to prepare vesicular stomatitis virus  (VSV) pseudotypes bearing various surface proteins  of HBV(HBsAg): S, M or L. The infectivities of the  psedotypes were determined by quantifying the number  of cells expressing green fluorescent protein (GFP).  The infectious unit (IU) of the pseudotype samples  neutralized with anti-VSV in the absence and  presence of anti-HBs S MAbs and titrated on HepG2  cells ranged from 1,000 to 4,000 IU\/ml and 200 to  400 IU\/ml, respectively. VSV pseudotype sample  bearing HBs M antigen showed the highest infectious  titer. This infectivity was inhibited by treatment  with lactoferrin or sulfated polysachharides.  Pretreatment of the cells with trypsin, tunicamycin  or DTT also inhibited the infectivity of the  pseudotypes. The VSV¿G*(HBV) pseudotypes might be  useful as a safe and rapid assay system to clarify  early steps of HBV infection.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783843366649\"\u003e\u003ch3\u003eA Rapid and Safe Assay System for Determination of HBV infectiousness\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783843366649","offer_id":54103115563333,"sku":"9783843366649","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/c0857e13-4a0f-4dbc-b0d6-278158d5092d.jpg?v=1769668493","url":"https:\/\/shop.autorenwelt.de\/products\/vsv-pseudotypes-bearing-hbsag-von-manujendra-saha","provider":"Autorenwelt Shop","version":"1.0","type":"link"}