{"product_id":"the-trypanocidal-effects-of-antibodies-to-t-brucei-recombinant-tubulin-von-david-kateete","title":"The trypanocidal effects of antibodies to T.brucei-recombinant tubulin","description":"\u003cp\u003eT. b. brucei alpha and beta tubulin genes were  expressed in E. coli using the pTrP2 expression  plasmid and 3-¿-Indoleacrylic acid as an inducer. The  expressed tubulins were analysed by SDS-PAGE and  confirmed by Western blotting, in which they had an  identical apparent molecular mass of 50kDa. The  expressed tubulins were individually dissolved in 8M  Urea and diluted 20-fold, after which they were  concentrated and extensively dialyzed against MES  buffer. Following dialysis, the average yield for  both alpha and beta tubulin was 0.7g per liter of  culture. Polyclonal antibodies were produced in each  of the 56 rabbits immunized with three doses of the  concentrated tubulins at either 150¿g or 450¿g per  immunization per animal. The effect of these  antibodies on T. b. brucei growth was studied by  culturing T. b. brucei bloodstream form in up to 25%  of the rabbit-raised antisera. The antisera from the  other rabbits did not inhibit T. b. brucei growth in  culture. These results show that antibodies to  bacterially expressed trypanosome tubulin may not be  cytotoxic to T. b. brucei.\u003c\/p\u003e\u003cdiv class=\"aw-variant-hidden-subtitle-div\" id=\"aw-variant-subtitle-9783838351407\"\u003e\u003ch3\u003eAn Evaluation of in Vitro Trypanocidal Activity of Antibodies to Recombinant Tubulin from  Trypanosoma B. Brucei\u003c\/h3\u003e\u003c\/div\u003e","brand":"Autorenwelt Shop","offers":[{"title":"Softcover - 9783838351407","offer_id":39485315645533,"sku":"9783838351407","price":49.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0940\/0622\/files\/859cc97a-29d1-4087-bbf6-d14c878fee7c.jpg?v=1773381782","url":"https:\/\/shop.autorenwelt.de\/products\/the-trypanocidal-effects-of-antibodies-to-t-brucei-recombinant-tubulin-von-david-kateete","provider":"Autorenwelt Shop","version":"1.0","type":"link"}